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InnovPlantProtect (InPP) was present at the conference “Building value together”, organized by our associate FNOP - National Association of Fruit and Vegetable Producers' Organizations.

InPP's executive director, António Saraiva, moderated the panel “Sustainability that generates value: The role of ESG in the future of the sector”, which included interventions from Catarina Pinto Correia (VdA), Cristina Câmara (APED), Filipa Saldanha (Crédito Agrícola), Joana Oom de Sousa (Sovena) and Rui Veríssimo Baptista (Companhia das Lezírias).

The opening session was given by Domingos dos Santos, president of FNOP and a member of CoLAB's Board of Directors of our CoLAB.

The meeting brought together producer organizations, farmers, companies, experts and political decision-makers to discuss the current challenges and look to the future of the national fruit and vegetable sector.

With the participation of national and international experts, the conference was a privileged space for sharing experiences and strategic reflection, focusing on the organization of production and the role of public policies in promoting sustainable growth.

Congratulations to FNOP for the initiative and the ability to bring together a panel of excellent speakers, making this conference a relevant and topical milestone for the sector.

Image credits: Voz do Campo magazine

FNOP Event

In viticulture, every little decision has an impact: on the soil, on the health of the plants and on the quality of the grapes that form the basis of the wine that reaches our table. The future of viticulture may depend on a single biosolution. Or a hundred. In VINNY, an ambitious European project of which InPP is a part, researchers from ten countries are looking for bioactives capable of curbing vine diseases - and, at the same time, reducing dependence on synthetic agrochemicals. What's at stake is not just science: it's the sustainability of this industry.

The aim of the VINNY project is simple but transformative: develop and implement effective, sustainable solutions and adaptable to the needs of winegrowers in various European countries, creating environmentally friendly biopesticides and biofertilizers, and advanced nano-encapsulation technologies, to reduce dependence on conventional chemicals and promote a healthier ecosystem and a better environment and a circular viticulture.

And at the heart of this mission is an essential cog in the wheel: the daily work of the researchers who search for answers invisible to the human eye - as is the case with Tiago Amaro, a researcher at InPP.

Image credits: VINNY Project

Searching for the Guardians of the Vine

The road to these new biosolutions begins in the field, with the vine. The initial work of Tiago Amaro, started in September 2024 and focuses on identifying and isolating microorganisms naturally present in the vines themselves, in samples received from partners in Portugal, Spain, Austria and Denmark.

From grapes, sticks or woody fragments, small microscopic worlds arrive in the laboratory that may contain the natural weapons needed to fighting three major threats to the vineyard, with a direct impact on farm profitability:
- A gray mold (Botrytis cinerea) and blue mold (Penicillium expansum): Fungi that cause post-harvest diseases, In the case of wine grapes, this affects the quality of the wine and makes it completely impossible to sell table grapes.
- The vine tumors: Caused by bacteria Allorhizobium vitis, This disease affects the plant in the field, causing leaf fall and reduced grape production.

Tiago Amaro, InnovPlantProtect researcher, identifying and isolating bacteria as part of the VINNY project. Image credits: InnovPlantProtect - Inês Ferreira

After isolating the microorganisms, Tiago dedicated himself to creating libraries of bacteria. What is a ‘Bacteria Library’? In the context of the investigation, a bacteria library is an organized and catalogued collection of bacteria isolated from different sources. It allows scientists to test each strain of bacteria against specific pathogens, constituting a vast catalog of potential biological ‘superheroes’ for plant protection.

This rigorous screening, which has already led to the analysis of more than 190 bacteria of this library is the first line of defense. The team selects the best candidates with the potential to be used as biological control agents against the diseases under study.

The Power of European Collaboration

What if the solution to protecting Portuguese vineyards is hidden in a Danish grape? Or in a bacterium isolated in Spain? One of the most exciting aspects of the project is its truly collaborative dimension, where researchers from ten countries are working in parallel, sharing answers, challenges and microorganisms in search of effective biosolutions for the whole of Europe.

All the solutions found will be shared, all the solutions will be tested by all the partners and it will be possible to build a ‘library of solutions’ against the various vine diseases“ emphasizes researcher Tiago Amaro.

The sharing of bacteria and extracts from different ecosystems (Portugal, Spain, Denmark and Austria) is crucial. An effective bacterium in Denmark could be the key to protecting Portuguese vineyards, and vice versa. This exchange of biological solutions, one of the innovative pillars of the project, makes it possible to exploit the microbial biodiversity beyond national borders. InPP has the fundamental role of testing, in grapes, the solutions discovered by our team as well as by other national and European partners.

This diversity of tests is a bet on the future: microorganisms that don't prove effective against vine diseases could be the solution for pathologies in other crops.

Left photo: Tiago Amaro, InPP researcher, observing a grapevine leaf, the target crop of the VINNY project, Right photo: Potted grapevine plants in the InPP greenhouse, ready to test the solutions found by the various VINNY partners. Image credits: InnovPlantProtect - Inês Ferreira

The Real Test: From the Lab to the Field

After selection in the laboratory, the next step - the formulation of the most promising bacteria - will be carried out in Portugal and Spain, at the University of Minho and the Polytechnic University of Catalonia. But it is in the field-testing phase that the greatest challenge of plant protection science lies, because even brilliant results in the laboratory can fail in the field. Formulation is the process that turns a bacterium into a product - stable, applicable and compatible with the farmer's needs.

Tiago Amaro emphasizes necessary resilience:

  • Field Uncertainty: Often, promising solutions in the laboratory or greenhouse are not as effective when applied in the field, due to environmental variables (climate, soil, etc.).
  • The Time Factor: Diseases such as Allorhizobium vitis may take a long time to develop, or the infection may not be relevant in certain years, which makes it difficult to obtain robust conclusions.
  • The Agricultural Cycle: It is necessary to test the formulation in the field during three to five consecutive years, and recording all the variations observed. With only one harvest a year, this process requires patience and persistence.

In total, from the discovery of a promising bacterium to the creation of a formulated product, proven to be effective and ready for the market, it can take around 10 years - a real test of any scientist's resilience.

Customized solutions: the new requirement of modern agriculture

The final challenge is to ensure that the tests are relevant to the producer's reality. The current trend in the agricultural sector is the search for customized solutions, adapted to the specific conditions of the farms: “There has to be a solution for every field and every farmer”, says the researcher.

This personalized approach requires more science, more rigor and more local knowledge - exactly what VINNY seeks to build.

A Europe united by science and the vine

InPP is part of this consortium, made up of 19 partners from ten countries, The project is led by the University of Minho and funded by the Horizon Europe program.

Together, they seek to answer a question that could shape the future of European viticulture: Will it be possible to find effective biosolutions for all partner countries?

The answer is still being written - in laboratories, in experimental vineyards, in fields in different climates and geographies.
And it's made up of small discoveries, many frustrations and a huge commitment to science.

Because protecting the vineyard of the future is not just a technical ambition.
It is a cultural, economic and environmental commitment.
And VINNY is helping to design that future - one microorganism at a time.

The final workshop highlighted three years of research dedicated to the early detection of pathogens in crops such as wheat and olive groves.

The project AlViGen has reached its final stretch, concluding three years of research focused on the genomic surveillance of agricultural diseases. The results now presented promise to strengthen the Alentejo agricultural sector's ability to respond to emerging phytosanitary threats.

On the day October 23rd, The final project workshop, The event brought together researchers, producers and technicians to share results and reflect on the future of genomic surveillance in Portuguese agriculture.

A pioneering genomic surveillance center

During AlViGen, the Alentejo's first genomic surveillance center, an infrastructure with capacity for early detection of diseases in strategic crops such as wheat and olive grove. This breakthrough marks a decisive step towards a more precise, sustainable and science-based agriculture.

Results and scientific contributions

Using innovative molecular tools, the project team succeeded:

  • Identify pathogenic fungi before visible symptoms appear on the plants;
  • Characterizing yellow rust strains, genetically linking them to others known at a global level;
  • Detecting resistance genes in wheat to the strains currently present in Portugal;
  • Developing diagnostic methods able to distinguish the different species of the fungus that causes gafa in olive groves.

During the workshop, the potential of the analysis of the airborne fungi community as a tool for early warning for multiple pathogens, allowing for more effective and preventive management of crop diseases.

From research to practical application

The event ended with a debate on how transform AlViGen results in a detection and warning service accessible to the agricultural sector. The initiative reflects the joint commitment between science, innovation and production, with a view to protecting national agriculture from the challenges of the future.

Partnerships and thanks

InnovPlantProtect would like to thank all the partners and funders of the project:
University of Évora, John Innes Centre, INIAV, De Prado, CERSUL, Eugénio de Almeida Foundation, Torre das Figueiras Estate, Almojanda, Malheiro Estate, Directorate-General for Food and Veterinary (DGAV), la Caixa“ Foundation”, BPI Bank e Foundation for Science and Technology (FCT).

Image credits: InnovPlantProtect - Inês Ferreira

EVENTS

Last Friday, August 26, the InPP received a visit from Catalonia's Councillor for External Action and Open Government, Victòria Alsina, and the delegate of the Government of Catalonia in Portugal, Rui Reis, They were accompanied by the Councillor of Elvas City Council, Paula Calado.

They were received by the heads of department, David Learmonth, iLaria Marengo e Sandra Correia, They presented InPP's infrastructures, laboratories and the different areas of research being explored by the collaborative laboratory's five departments.

The aim of this visit was to create and establish new partnerships and collaborations with this autonomous community of Spain, a key international player for Portugal in its economic and scientific spheres, in which the agri-food industries play a major role. Establishing partnerships is part of InPP's philosophy and, therefore, this visit allowed us to explore the strengths of each of those involved and find points of synergy, where we wanted to identify areas of interest for possible partnerships and collaborations.

During the visit to InPP's facilities, the delegation also had the opportunity to speak to some of InPP's researchers, who were able to personally explain some of the research they are currently carrying out at InPP in the fight against emerging plant pests and diseases.

InnovPlantProtect (InPP) team identifies the most promising materials for encapsulating biological protection agents to control emerging pests and diseases in agricultural crops. Natural products of animal origin, from marine and terrestrial sources, as well as synthetic ones, have been the most widely used due to their low toxicity and biodegradability, the researchers conclude in a systematic review article now published in the scientific journal ACS Agricultural Science & Technology. But are these solutions scalable and economically viable?

Biological protection agents, microorganisms such as bacteria, fungi or biomolecules with active substances capable of preventing or controlling or suppressing pests and diseases in plants, have been considered more sustainable alternatives to traditional chemical pesticides. However, these agents are very sensitive to atmospheric conditions and begin to degrade due to humidity, temperature and solar radiation.

Due to the sensitivity of the active substances, the challenge is to develop a biodegradable and sustainable material that envelops, i.e. encapsulates the biological agents, protecting them so that they can be applied effectively to Mediterranean agricultural crops. The encapsulation of biological agents brings several advantages for the agricultural producer, namely ease of handling, controlled application of lower and less frequent dosages, greater specificity for the target, prolonged stability and maximized permanence, which leads to greater effectiveness of the biological agent in combating pests and diseases.

Schematic representation of the encapsulation process of a biological agent to protect pear pests

The team, from Formulations and Process Development Department of the InPP, reviewed 98 scientific articles, published between 2013 and 2022, and concluded that “micro and nanoencapsulation of biological protection agents using materials from marine, terrestrial or synthetic sources should provide agricultural producers with access to safer and more sustainable plant protection products”. The review article [1], entitled Micro- and Nanocarriers for Encapsulation of Biological Plant Protection Agents: A Systematic Literature Review, has just been published in the scientific journal ACS Agricultural Science & Technology.

The four researchers analyzed the studies with a view to “identifying the materials most commonly used to encapsulate biological agents for pest and disease control with greater efficacy, greater systemic activity and less environmental impact”. The researchers also analyzed the encapsulation methods and techniques currently being used by research teams in various parts of the world.

“The data presented in this article indicate that materials based on polymers, of natural or synthetic origin, and inorganic materials can improve the stability and performance of a wide range of bioinspired active substances,” say the researchers.

However, the team warns that “although research interest in these encapsulating materials is increasing, the current level of knowledge does not yet allow for a totally fair and unbiased assessment of the pros and cons that will arise from the use of micro and nano systems for encapsulating biological agents and their use in agriculture”, adding that “a better understanding of the fate and long-term safety of these products is needed”.

“Although the technologies presented in this review exhibit promising efficacy and safety profiles, it is unlikely that all of them will be scalable and transformable into economically viable solutions to current and future agricultural problems. More research and development of efforts against crop pests and diseases is needed, focusing on strategies that truly take into account the needs of farmers in agricultural fields, as only then will innovation be possible,” the team concludes.

Researchers Cláudia Silva, Tânia Pinto, Sónia Siquenique and David Learmonth

Original article

ACS Agricultural Science & Technology Journal

Micro- and Nanocarriers for Encapsulation of Biological Plant Protection Agents: A Systematic Literature Review

Tânia Pinto, Cláudia Silva, Sónia Siquenique and David Learmonth

DOI: https://doi.org/10.1021/acsagscitech.2c00113

[1] In a review article, researchers critically summarize what has already been studied and published by other scientists.

On August 18th, researchers from the New Biopesticides Department of InnovPlantProtect (InPP).., Pedro Rosa e Tiago Amaro, were in the Tagus/Sorraia basin, in Coruche and Porto Alto, in Samora Correia, to collect samples of rice infected with the fungus Magnaporthe oryzae, which causes pyriculariosis. The sampling was carried out as part of the BlaSTOP project - Developing integrated solutions to combat rice pyriculariosis.

As early as August 1st, the team had been to the Mondego basin, in Montemor-o-Velho, to take samples.

The BlaSTOP project aims to develop a sustainable and effective solution for controlling rice pyriculariosis caused by this fungus. The project has the support of COTArroz - Operational and Technological Center for Rice and Casa do Arroz, in the Tagus basin, and the Aparroz - Agrupamento de Produtores de Arroz do Vale do Sado Lda, in the Sado basin, where the team hopes to collect in the near future.

Sampling in the Tagus area, in Coruche and Porto Alto