engenharia genética – InnovPlantProtect

NEWS

The Science that Grows from the Vine: How Biosolutions Can Protect the Wine of the Future

November 28, 2025
Blog, Highlight, News, Project, Report
Ines Ferreira

In viticulture, every little decision has an impact: on the soil, on the health of the plants and on the quality of the grapes that form the basis of the wine that reaches our table. The future of viticulture may depend on a single biosolution. Or a hundred. In VINNY, an ambitious European project of which InPP is a part, researchers from ten countries are looking for bioactives capable of curbing vine diseases - and, at the same time, reducing dependence on synthetic agrochemicals. What's at stake is not just science: it's the sustainability of this industry.

The aim of the VINNY project is simple but transformative: develop and implement effective, sustainable solutions and adaptable to the needs of winegrowers in various European countries, creating environmentally friendly biopesticides and biofertilizers, and advanced nano-encapsulation technologies, to reduce dependence on conventional chemicals and promote a healthier ecosystem and a better environment and a circular viticulture.

And at the heart of this mission is an essential cog in the wheel: the daily work of the researchers who search for answers invisible to the human eye - as is the case with Tiago Amaro, a researcher at InPP.

Image credits: VINNY Project

Searching for the Guardians of the Vine

The road to these new biosolutions begins in the field, with the vine. The initial work of Tiago Amaro, started in September 2024 and focuses on identifying and isolating microorganisms naturally present in the vines themselves, in samples received from partners in Portugal, Spain, Austria and Denmark.

From grapes, sticks or woody fragments, small microscopic worlds arrive in the laboratory that may contain the natural weapons needed to fighting three major threats to the vineyard, with a direct impact on farm profitability:
- A gray mold (Botrytis cinerea) and blue mold (Penicillium expansum): Fungi that cause post-harvest diseases, In the case of wine grapes, this affects the quality of the wine and makes it completely impossible to sell table grapes.
- The vine tumors: Caused by bacteria Allorhizobium vitis, This disease affects the plant in the field, causing leaf fall and reduced grape production.

Tiago Amaro, InnovPlantProtect researcher, identifying and isolating bacteria as part of the VINNY project. Image credits: InnovPlantProtect - Inês Ferreira

After isolating the microorganisms, Tiago dedicated himself to creating libraries of bacteria. What is a ‘Bacteria Library’? In the context of the investigation, a bacteria library is an organized and catalogued collection of bacteria isolated from different sources. It allows scientists to test each strain of bacteria against specific pathogens, constituting a vast catalog of potential biological ‘superheroes’ for plant protection.

This rigorous screening, which has already led to the analysis of more than 190 bacteria of this library is the first line of defense. The team selects the best candidates with the potential to be used as biological control agents against the diseases under study.

The Power of European Collaboration

What if the solution to protecting Portuguese vineyards is hidden in a Danish grape? Or in a bacterium isolated in Spain? One of the most exciting aspects of the project is its truly collaborative dimension, where researchers from ten countries are working in parallel, sharing answers, challenges and microorganisms in search of effective biosolutions for the whole of Europe.

“All the solutions found will be shared, all the solutions will be tested by all the partners and it will be possible to build a ‘library of solutions’ against the various vine diseases“ emphasizes researcher Tiago Amaro.

The sharing of bacteria and extracts from different ecosystems (Portugal, Spain, Denmark and Austria) is crucial. An effective bacterium in Denmark could be the key to protecting Portuguese vineyards, and vice versa. This exchange of biological solutions, one of the innovative pillars of the project, makes it possible to exploit the microbial biodiversity beyond national borders. InPP has the fundamental role of testing, in grapes, the solutions discovered by our team as well as by other national and European partners.

This diversity of tests is a bet on the future: microorganisms that don't prove effective against vine diseases could be the solution for pathologies in other crops.

Left photo: Tiago Amaro, InPP researcher, observing a grapevine leaf, the target crop of the VINNY project, Right photo: Potted grapevine plants in the InPP greenhouse, ready to test the solutions found by the various VINNY partners. Image credits: InnovPlantProtect - Inês Ferreira

The Real Test: From the Lab to the Field

After selection in the laboratory, the next step - the formulation of the most promising bacteria - will be carried out in Portugal and Spain, at the University of Minho and the Polytechnic University of Catalonia. But it is in the field-testing phase that the greatest challenge of plant protection science lies, because even brilliant results in the laboratory can fail in the field. Formulation is the process that turns a bacterium into a product - stable, applicable and compatible with the farmer's needs.

Tiago Amaro emphasizes necessary resilience:

Field Uncertainty: Often, promising solutions in the laboratory or greenhouse are not as effective when applied in the field, due to environmental variables (climate, soil, etc.).
The Time Factor: Diseases such as Allorhizobium vitis may take a long time to develop, or the infection may not be relevant in certain years, which makes it difficult to obtain robust conclusions.
The Agricultural Cycle: It is necessary to test the formulation in the field during three to five consecutive years, and recording all the variations observed. With only one harvest a year, this process requires patience and persistence.

In total, from the discovery of a promising bacterium to the creation of a formulated product, proven to be effective and ready for the market, it can take around 10 years - a real test of any scientist's resilience.

Customized solutions: the new requirement of modern agriculture

The final challenge is to ensure that the tests are relevant to the producer's reality. The current trend in the agricultural sector is the search for customized solutions, adapted to the specific conditions of the farms: “There has to be a solution for every field and every farmer”, says the researcher.

This personalized approach requires more science, more rigor and more local knowledge - exactly what VINNY seeks to build.

A Europe united by science and the vine

InPP is part of this consortium, made up of 19 partners from ten countries, The project is led by the University of Minho and funded by the Horizon Europe program.

Together, they seek to answer a question that could shape the future of European viticulture: Will it be possible to find effective biosolutions for all partner countries?

The answer is still being written - in laboratories, in experimental vineyards, in fields in different climates and geographies.
And it's made up of small discoveries, many frustrations and a huge commitment to science.

Because protecting the vineyard of the future is not just a technical ambition.
It is a cultural, economic and environmental commitment.
And VINNY is helping to design that future - one microorganism at a time.

AlViGen project strengthens genomic surveillance of agricultural diseases in Alentejo

October 24, 2025
Blog, Highlight, News, Project
Ines Ferreira

The final workshop highlighted three years of research dedicated to the early detection of pathogens in crops such as wheat and olive groves.

The project AlViGen has reached its final stretch, concluding three years of research focused on the genomic surveillance of agricultural diseases. The results now presented promise to strengthen the Alentejo agricultural sector's ability to respond to emerging phytosanitary threats.

On the day October 23rd, The final project workshop, The event brought together researchers, producers and technicians to share results and reflect on the future of genomic surveillance in Portuguese agriculture.

A pioneering genomic surveillance center

During AlViGen, the Alentejo's first genomic surveillance center, an infrastructure with capacity for early detection of diseases in strategic crops such as wheat and olive grove. This breakthrough marks a decisive step towards a more precise, sustainable and science-based agriculture.

Results and scientific contributions

Using innovative molecular tools, the project team succeeded:

Identify pathogenic fungi before visible symptoms appear on the plants;
Characterizing yellow rust strains, genetically linking them to others known at a global level;
Detecting resistance genes in wheat to the strains currently present in Portugal;
Developing diagnostic methods able to distinguish the different species of the fungus that causes gafa in olive groves.

During the workshop, the potential of the analysis of the airborne fungi community as a tool for early warning for multiple pathogens, allowing for more effective and preventive management of crop diseases.

From research to practical application

The event ended with a debate on how transform AlViGen results in a detection and warning service accessible to the agricultural sector. The initiative reflects the joint commitment between science, innovation and production, with a view to protecting national agriculture from the challenges of the future.

Partnerships and thanks

InnovPlantProtect would like to thank all the partners and funders of the project:
University of Évora, John Innes Centre, INIAV, De Prado, CERSUL, Eugénio de Almeida Foundation, Torre das Figueiras Estate, Almojanda, Malheiro Estate, Directorate-General for Food and Veterinary (DGAV), la Caixa“ Foundation”, BPI Bank e Foundation for Science and Technology (FCT).

Image credits: InnovPlantProtect - Inês Ferreira

InPP was in Italy for the kick-off meeting of the European PROSPER project

October 6, 2025
Blog, Highlight, News, Project
Ines Ferreira

InPP took part in the kick-off meeting for the European PROSPER project, held on October 2 and 3 in Pavia, Italy. In attendance were the director of the Monitoring and Diagnostics Department, Ilaria Marengo, and the project manager, Bruno Orrico.

PROSPER's main objective is to transform European agriculture by valorizing highly resilient “orphan” legumes - forgotten crops, but full of potential to face the climate and food challenges of the future.

The project promotes sustainable, innovative practices adapted to different agricultural realities.

Over the two days, 27 partners from 13 countries met for presentations, in-depth discussions and strategic talks about the project's next steps.

We are excited about what comes next, certain that this journey will be more than a collaboration - it will be a true cooperation within an exceptional team.

Join us and keep up to date with all the news from the PROSPER Project!

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EVENTS

The talking chestnut trees arrive at the Sabugal School Group

November 9, 2021
Blog, Project
Ines Ferreira

The project “Educating to know, protect and monitor chestnut trees through IoT technology”, The project, co-financed by the Environmental Fund, was presented last week to 12th grade students from the Sabugal School Group.

Around 50 Biology, Technology and Tourism students spent part of the morning and afternoon of November 4 with the InnovPlantProtect (InPP) team, in order to understand the importance of protecting trees, and in particular chestnut trees, a local natural heritage. The project, led by InPP, is directed by Sabugal Town Hall (CMS).

The InPP-led project was presented to students...

The day was an Education 4.0 experience, in which traditional subjects such as Biology and Ecology were integrated with a description and explanation of the application of “Internet of Things” (IoT) technology to tree monitoring. Incidentally, the presentation coincided with the COP 26 Climate Summit, which ties in perfectly with the main issues being discussed in Glasgow, Scotland.

In the afternoon, the pupils were taken to a meadow used as a study area by the InPP and CMS team. It was there that they were able to see and understand first-hand how IoT sensors work. The visit to the grove was an opportunity for them to take part in practical exercises and learn how to measure some of the physical parameters of trees, such as height, diameter and crown width using traditional methods, while at the same time seeing more modern tools in action.

Sampling and molecular identification of yellow rust in Portugal

November 9, 2021
Blog
Ines Ferreira

With in-depth knowledge of the strains and breeds that exist in the country, the Elvas CoLAB can thus devise more efficient and targeted combat strategies.

InnovPlantProtect (InPP) is analyzing yellow rust samples collected from wheat fields in Portugal. Identification at InPP is carried out using PCR techniques and analysis of DNA fragments using SeqStudio, which makes it possible to determine the lineage of the fungus by examining molecular markers.

The polymerase chain reaction, or PCR, is a technique that allows you to copy a specific region of DNA, in vitro. SeqStudio is a sequencing machine that also allows the length of fragments to be measured, from which it is possible to correlate the size of different molecular markers with a given lineage.

Yellow rust is a disease caused by the fungus Puccinia striiformis f. sp. tritici, This pathogen, which affects wheat and other cereals all over the world, jeopardizes the future and stability of cereal crops. This pathogen is biologically very dynamic, adapting quickly to climate change and to the resistant cereal varieties that are being introduced onto the market.

A. Yellow rust on wheat; B. Molecular markers amplified by PCR on an agarose gel; C. Fragment analysis to determine the size of the markers and establish the fungal lineage

So far, the PstS10 strain, the most prevalent in Europe, has been identified. PstS10 has proved to be very aggressive, affecting wheat varieties that were previously resistant to yellow rust. The race of this strain was determined by RustWatch in Denmark to be Benchmark. InPP sent samples to this European project to combat wheat rusts, which, among many other aspects, also involves genotyping and identifying the strains and races of the fungus that causes yellow rust.

With in-depth knowledge of the strains and races present in Portugal, the InPP can therefore devise more efficient and targeted combat strategies, by improving wheat varieties resistant to yellow rust and developing bioinspired control agents.

InnovPlantProtect's project to combat this disease was started in March 2020.

Puglia warns farmers about ineffective cures for Xylella fastidiosa

November 3, 2021
Blog
Ines Ferreira

No product currently on the market is capable of eliminating Xylella fastidiosa, the Commission for the Regional Budget of Puglia, southern Italy. The bacterium continues to seriously affect olive groves in Italy's main olive oil producing region.

“The two products marketed in recent weeks as a treatment against the drying out of trees, and useful for bringing plants back to their original glory, are a mixture of natural soaps or adjuvants,” said the director of Puglia's phytosanitary observatory, Salvatore Infantino, at a public hearing, quoted by OliveOilTimes. The heads of the regional budget commission for this area of southern Italy, which forms the “heel” of the “boot”, guarantee that there are currently no products on the market capable of eliminating the bacteria Xylella fastidiosa of infected trees, particularly olive trees.

This bacterium is the most active pathogen affecting olive trees in Italy's main olive oil producing region. For Salvatore Infantino, the development of a product capable of combating it remains an important goal for many of those striving to reduce the devastation caused by the bacteria. Xylella fastidiosa.

Public authorities must not make the mistakes of the past by giving credence to unscientific theories that have already caused so much damage and wasted so much time.
Fabiano Amati, president of Puglia's regional budget committee

A Xylella fastidiosa was first detected in the European Union precisely in Puglia, in October 2013, and was responsible for an outbreak that, by 2015, had already infected one million olive trees in this Italian region alone. “Currently, good agricultural practices and eradication are the only weapons we have against the Xylella, It's a way of saving time while we wait for a truly effective treatment,” says Salvatore Infantino.