InnovPlantProtect (InPP) was present at the conference “Building value together”, organized by our associate FNOP - National Association of Fruit and Vegetable Producers' Organizations.
InPP's executive director, António Saraiva, moderated the panel “Sustainability that generates value: The role of ESG in the future of the sector”, which included interventions from Catarina Pinto Correia (VdA), Cristina Câmara (APED), Filipa Saldanha (Crédito Agrícola), Joana Oom de Sousa (Sovena) and Rui Veríssimo Baptista (Companhia das Lezírias).
The opening session was given by Domingos dos Santos, president of FNOP and a member of CoLAB's Board of Directors of our CoLAB.
The meeting brought together producer organizations, farmers, companies, experts and political decision-makers to discuss the current challenges and look to the future of the national fruit and vegetable sector.
With the participation of national and international experts, the conference was a privileged space for sharing experiences and strategic reflection, focusing on the organization of production and the role of public policies in promoting sustainable growth.
Congratulations to FNOP for the initiative and the ability to bring together a panel of excellent speakers, making this conference a relevant and topical milestone for the sector.
In viticulture, every little decision has an impact: on the soil, on the health of the plants and on the quality of the grapes that form the basis of the wine that reaches our table. The future of viticulture may depend on a single biosolution. Or a hundred. In VINNY, an ambitious European project of which InPP is a part, researchers from ten countries are looking for bioactives capable of curbing vine diseases - and, at the same time, reducing dependence on synthetic agrochemicals. What's at stake is not just science: it's the sustainability of this industry.
The aim of the VINNY project is simple but transformative: develop and implement effective, sustainable solutions and adaptable to the needs of winegrowers in various European countries, creating environmentally friendly biopesticides and biofertilizers, and advanced nano-encapsulation technologies, to reduce dependence on conventional chemicals and promote a healthier ecosystem and a better environment and a circular viticulture.
And at the heart of this mission is an essential cog in the wheel: the daily work of the researchers who search for answers invisible to the human eye - as is the case with Tiago Amaro, a researcher at InPP.
Image credits: VINNY Project
Searching for the Guardians of the Vine
The road to these new biosolutions begins in the field, with the vine. The initial work of Tiago Amaro, started in September 2024 and focuses on identifying and isolating microorganisms naturally present in the vines themselves, in samples received from partners in Portugal, Spain, Austria and Denmark.
From grapes, sticks or woody fragments, small microscopic worlds arrive in the laboratory that may contain the natural weapons needed to fighting three major threats to the vineyard, with a direct impact on farm profitability: - A gray mold (Botrytis cinerea) and blue mold (Penicillium expansum): Fungi that cause post-harvest diseases, In the case of wine grapes, this affects the quality of the wine and makes it completely impossible to sell table grapes. - The vine tumors: Caused by bacteria Allorhizobium vitis, This disease affects the plant in the field, causing leaf fall and reduced grape production.
Tiago Amaro, InnovPlantProtect researcher, identifying and isolating bacteria as part of the VINNY project. Image credits: InnovPlantProtect - Inês Ferreira
After isolating the microorganisms, Tiago dedicated himself to creating libraries of bacteria. What is a ‘Bacteria Library’? In the context of the investigation, a bacteria library is an organized and catalogued collection of bacteria isolated from different sources. It allows scientists to test each strain of bacteria against specific pathogens, constituting a vast catalog of potential biological ‘superheroes’ for plant protection.
This rigorous screening, which has already led to the analysis of more than 190 bacteria of this library is the first line of defense. The team selects the best candidates with the potential to be used as biological control agents against the diseases under study.
The Power of European Collaboration
What if the solution to protecting Portuguese vineyards is hidden in a Danish grape? Or in a bacterium isolated in Spain? One of the most exciting aspects of the project is its truly collaborative dimension, where researchers from ten countries are working in parallel, sharing answers, challenges and microorganisms in search of effective biosolutions for the whole of Europe.
“All the solutions found will be shared, all the solutions will be tested by all the partners and it will be possible to build a ‘library of solutions’ against the various vine diseases“ emphasizes researcher Tiago Amaro.
The sharing of bacteria and extracts from different ecosystems (Portugal, Spain, Denmark and Austria) is crucial. An effective bacterium in Denmark could be the key to protecting Portuguese vineyards, and vice versa. This exchange of biological solutions, one of the innovative pillars of the project, makes it possible to exploit the microbial biodiversity beyond national borders. InPP has the fundamental role of testing, in grapes, the solutions discovered by our team as well as by other national and European partners.
This diversity of tests is a bet on the future: microorganisms that don't prove effective against vine diseases could be the solution for pathologies in other crops.
Left photo: Tiago Amaro, InPP researcher, observing a grapevine leaf, the target crop of the VINNY project, Right photo: Potted grapevine plants in the InPP greenhouse, ready to test the solutions found by the various VINNY partners. Image credits: InnovPlantProtect - Inês Ferreira
The Real Test: From the Lab to the Field
After selection in the laboratory, the next step - the formulation of the most promising bacteria - will be carried out in Portugal and Spain, at the University of Minho and the Polytechnic University of Catalonia. But it is in the field-testing phase that the greatest challenge of plant protection science lies, because even brilliant results in the laboratory can fail in the field. Formulation is the process that turns a bacterium into a product - stable, applicable and compatible with the farmer's needs.
Tiago Amaro emphasizes necessary resilience:
Field Uncertainty: Often, promising solutions in the laboratory or greenhouse are not as effective when applied in the field, due to environmental variables (climate, soil, etc.).
The Time Factor: Diseases such as Allorhizobium vitis may take a long time to develop, or the infection may not be relevant in certain years, which makes it difficult to obtain robust conclusions.
The Agricultural Cycle: It is necessary to test the formulation in the field during three to five consecutive years, and recording all the variations observed. With only one harvest a year, this process requires patience and persistence.
In total, from the discovery of a promising bacterium to the creation of a formulated product, proven to be effective and ready for the market, it can take around 10 years - a real test of any scientist's resilience.
Customized solutions: the new requirement of modern agriculture
The final challenge is to ensure that the tests are relevant to the producer's reality. The current trend in the agricultural sector is the search for customized solutions, adapted to the specific conditions of the farms: “There has to be a solution for every field and every farmer”, says the researcher.
This personalized approach requires more science, more rigor and more local knowledge - exactly what VINNY seeks to build.
A Europe united by science and the vine
InPP is part of this consortium, made up of 19 partners from ten countries, The project is led by the University of Minho and funded by the Horizon Europe program.
Together, they seek to answer a question that could shape the future of European viticulture: Will it be possible to find effective biosolutions for all partner countries?
The answer is still being written - in laboratories, in experimental vineyards, in fields in different climates and geographies. And it's made up of small discoveries, many frustrations and a huge commitment to science.
Because protecting the vineyard of the future is not just a technical ambition. It is a cultural, economic and environmental commitment. And VINNY is helping to design that future - one microorganism at a time.
The final workshop highlighted three years of research dedicated to the early detection of pathogens in crops such as wheat and olive groves.
The project AlViGen has reached its final stretch, concluding three years of research focused on the genomic surveillance of agricultural diseases. The results now presented promise to strengthen the Alentejo agricultural sector's ability to respond to emerging phytosanitary threats.
On the day October 23rd, The final project workshop, The event brought together researchers, producers and technicians to share results and reflect on the future of genomic surveillance in Portuguese agriculture.
A pioneering genomic surveillance center
During AlViGen, the Alentejo's first genomic surveillance center, an infrastructure with capacity for early detection of diseases in strategic crops such as wheat and olive grove. This breakthrough marks a decisive step towards a more precise, sustainable and science-based agriculture.
Results and scientific contributions
Using innovative molecular tools, the project team succeeded:
Identify pathogenic fungi before visible symptoms appear on the plants;
Characterizing yellow rust strains, genetically linking them to others known at a global level;
Detecting resistance genes in wheat to the strains currently present in Portugal;
Developing diagnostic methods able to distinguish the different species of the fungus that causes gafa in olive groves.
During the workshop, the potential of the analysis of the airborne fungi community as a tool for early warning for multiple pathogens, allowing for more effective and preventive management of crop diseases.
From research to practical application
The event ended with a debate on how transform AlViGen results in a detection and warning service accessible to the agricultural sector. The initiative reflects the joint commitment between science, innovation and production, with a view to protecting national agriculture from the challenges of the future.
Partnerships and thanks
InnovPlantProtect would like to thank all the partners and funders of the project: University of Évora, John Innes Centre, INIAV, De Prado, CERSUL, Eugénio de Almeida Foundation, Torre das Figueiras Estate, Almojanda, Malheiro Estate, Directorate-General for Food and Veterinary (DGAV), la Caixa“ Foundation”, BPI Bank e Foundation for Science and Technology (FCT).
Discover the latest advances in project WhYRust, regarding fungal epidemiology and population structure studies, data collection and model validation of a warning system, and identification of wheat genes involved in YR resistance.
Supporting the Portuguese wheat breeding program in the climate change scenario is the purpose of the WhYRust project. Yellow rust (YR), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases for wheat (Triticum ssp.), with global annual losses of US$ 979 million. Planting resistant cultivars is the most effective, economic, and environmental-friendly way to control diseases. Among other things, WhYRust aims to identify the wheat's genome areas associated with resistance or susceptibility to YR, a major threat to this cereal.
InnovPlantProtect, namely through its Department of Protection of Specific Crops - Department 2, leads this project, with the participation of INIAV Elvas and ITQB NOVA, and the collaboration of ANPOC and CERSUL.We are (1) studying the fungus epidemiology in Portugal, evaluating the fungal pathogenesis mechanisms by (2) sequencing the fungus genome, (3) investigating its transcriptome during plant interaction and (4) proteome of secreted proteins under conditions of low versus high temperature, (5) performing genome-wide association studies to identify resistance genes in the plant, and (6) developing genomic prediction models through machine learning in order to support precision breeding.
Wheat seeds germination in vitro. Two-week-old wheat plants in our growth chamber. Sampling of yellow rust fungal spores from wheat leaf showing symptoms of YR disease (by Miguel Teixeira and Cláudia Rato)
As far as fungal epidemiology and population structure studies are concerned, the quarantine enforced by COVID-19 in the first trimester of 2020 imposed some difficulties, but some wheat infected leaves were sampled in the 2019/2020 season. New sampling will be performed for the 2020/2021 season. Collected spores are being used to isolate single pustules, which will be further used to identify the specific fungus races.
Germination of YR fungus spores (called urediniospores) in vitro conditions (by Miguel Teixeira and Cláudia Rato)
Sampling app, by iLaria Marengo
InPP's Department 2 is also collaborating with Department 4 (Monitoring and Diagnosis). Portuguese agronomists are going to be trained on data collection and model validation of a yellow rust warning system. The goal is to use Open Data Kit (ODK), an open source software for (android) mobile devices that uses a form designed by InPP (Dep. 4) to collect data in the field and offline. The submission of the data to the InPP server is made once back to the office, when Internet connectivity is available. The app not only speeds up the data collection but also improves its management and accessibility.
Regarding the identification of wheat genes involved in YR resistance, the first field trial for the evaluation of disease symptoms in a panel of 250 wheat accessions of INIAV's breeding pool has been sown in the INIAV Elvas field. The crop of common or bread wheat (Triticum aestivum) is checked by the team every week, to remove weeds and check accessions status.
Disease evaluation will begin as soon as first symptoms develop. Image analysis of infected leaves will be performed to identify the percentage of pustules per leaf. This experiment will be performed in three consecutive years, and the data will be used to access the genetic and phenotypic variability within this panel and for future genome-wide association studies.
Field work by researchers Miguel Teixeira, Asmae Jlilat, Ana Miguel Reis, Diana Acácio, Flavio Storino, Diana Sousa and Hadi Sheikhnejad
For 2021, we also expect the most prevalent YR fungus race will have its genome sequenced and potential virulent factors will be identified by in silico analysis.
Accelerating technological innovation and creating new sustainable solutions for the protection of agricultural crops against pests, diseases and weeds, while protecting ecosystems, is the aim of the new collaboration between Syngenta Crop Protection, an InnovPlantProtect (InPP) member, and Insilico Medicine, which specializes in artificial intelligence (AI) and deep learning.
The partnership “will further transform agriculture by providing farmers around the world with the tools they need to produce healthy, nutritious, affordable and sustainably grown food in the most efficient way, while minimizing environmental impact,” Camilla Corsi said in a statement, head of Crop Protection Research at Syngenta.
Insilico Medicine's solid and proven experience in the use of AI and deep learning to develop, synthesize and validate new active substances promises to “transform the development of new solutions that help keep plants safe, from planting to harvest,” Syngenta points out. The safety of agricultural products and the protection of human health, in the short and long term, are also top priorities.
Twelve founding members, the InnovPlantProtect team and all those who contributed to giving birth to this young CoLab are to be congratulated today: two years ago, the formal constitution of InPP was registered with a notary in Elvas.
On January 24, 2019, in pre-pandemic times, the public deed was signed to establish the InnovPlantProtect - Associação collaborative laboratory in Elvas. Today, a highly qualified team is now complete, work is underway and projects are in progress, marking the second anniversary of the formal establishment of the collaborative laboratory, which has twelve founding partners.
Despite the restrictions imposed on all of us by the Covid-19 crisis, 2021 promises to be auspicious. “The conditions are beginning to be created for the careful and dedicated management to achieve the desired results,” says the mayor of Elvas (CME), an InnovPlantProtect (InPP) member. “Now it's ‘hands on’,” says Nuno Mocinha.
Admitting that “it's been a Herculean task to set up the CoLab”, the chairman of the board of Casa do Arroz has no doubt that “it's been worth it” and that InPP “will be on the map”. “It has everything it takes to succeed,” assures Pedro Monteiro. “For us, the CoLab is our insurance for the future. It's a laboratory that represents a great opportunity for the defense of rice cultivation in Portugal.”
InPP members sign the public deed of incorporation, on January 24, 2019, at the notary's office in Elvas.
The traditional “family photo”.
The great satisfaction with the quality of the technical staff is shared by CME, CEBAL and Fertiprado - and by InPP's executive director and “man at the helm”, Pedro Fevereiro. “The high level of professionalism, the dedication of all the professionals who make it up and its great organization put this institution on the map of major international organizations in the field of plant protection,” says Liliana Marum, a researcher at CEBAL. “CEBAL, as a founding partner, congratulates InnovPlantProtect on its excellent work and its mission of great importance for the sustainability of national agriculture.”.
“Although it's still in the installation phase, the excellent team already set up by Professor Pedro Fevereiro gives us high hopes for the success of the important projects already underway in the InnovPlantProtect collaborative laboratory,” says Ana Barradas, Fertiprado's R&D director. The company is already working with InPP on the Pythium project, the aim of which is to “identify the pathological organism that causes root rot and find a solution to eliminate it”.
“The CoLab is our insurance for the future.” Pedro Monteiro, Casa do Arroz
“Fertiprado is very proud to be a founding member of the InnovPlantProtect CoLab and we are very pleased to be celebrating its second anniversary,” concludes Ana Barradas. “After two years, the CoLab has a team of 38 employees, the vast majority of whom are highly educated, highly motivated and competent. It's a team that is expected to make a difference on the agricultural innovation scene and to be able to respond to the challenge of developing biopesticides and services that reduce the impact of pests and diseases on Mediterranean crops,” stresses Pedro Fevereiro.
“Our resilience lies and will lie in the fact that we have created a friendly and interactive working environment, which we want to maintain and expand,” argues InPP's CEO, who bet on this project “because it is a way of participating in the transfer of academic knowledge to real life, on the one hand, and of participating in the construction of solutions for a more productive and sustainable agriculture, on the other.”.
For Pedro Fevereiro, the second anniversary of the establishment of CoLab means above all that the institution has survived the initial installation phase, “which is not over yet, even despite the pandemic situation we are living in”. “It means that what we invested in is still in a position to progress and develop. The preliminary process of setting up was the effort of a team that knew how to put the common interest ahead of individual agendas and that knew how to find a way to make this project viable,” stresses the executive director.
“CoLab has a team of 38 highly motivated and competent employees.” Pedro Fevereiro, CEO of InPP
In the current context, the biggest challenge “is to keep the team active and working, while we wait for the possibility of having everyone working in the same place and in suitable conditions to develop the projects we already have in hand” - a goal that Pedro Fevereiro hopes to achieve within six months. “Another challenge is to create a modern institution of reference in a territory far from the major centers.” For Nuno Mocinha, InnovPlantProtect represents, in fact, “a qualitative leap in the affirmation of Elvas as a research hub in the national context and a special partnership between academia, companies and local government”.
The 38 members of the InnovPlantProtect team in January 2021.
The main objective now is to “develop national and international projects in the field of crop protection that have the capacity to present solutions as soon as possible, while guaranteeing the CoLab's financial balance,” says Pedro Fevereiro. New bio-based solutions for protecting plants against pests and diseases are “a market that is expected to double in the next five years at international level and which will certainly expand enormously in the European Union in the light of the European Green Deal and the ‘Farm to Fork’ and ‘Biodiversity’ Strategies”, recalls Felisbela Torres de Campos, responsible for Regulatory and Business Sustainability at Syngenta Portugal, an InPP member.
“This collaborative laboratory is on the right track: towards the sustainability of agriculture,” says Felisbela Campos, who considers CoLab “a strategic partnership for Syngenta and also because of the company's strong commitment to R&D in biological solutions for agriculture”. In addition, “InnovPlantProtect positions Portugal as a creator of technologies to solve specific phytosanitary problems in Portuguese agriculture, which will certainly help to reduce the country's dependence on foreign technologies and ensure that the solutions found are more effective from the outset”.
“We welcome this partnership and its potential to accelerate innovationfor farmers and nature”. Felisbela Torres de Campos, Syngenta Portugal
“CEBAL, as a founding partner, congratulates InnovPlantProtect for its excellent work and its mission of great importance for the sustainability of national agriculture,” agrees Liliana Marum, a researcher at the biotechnology center based in Beja.
InPP's founding partners are the National Institute for Agricultural and Veterinary Research, Elvas City Council, the New University of Lisbon, Syngenta Crop Protection, the Alentejo Agricultural and Agri-Food Biotechnology Center, Casa do Arroz, Bayer Crop Science, the University of Évora, Fertiprado, the National Federation of Fruit and Vegetable Producers' Organizations, the National Association of Protein, Oilseed and Cereal Producers, and the National Association of Maize and Sorghum Producers.
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