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InnovPlantProtect (InPP) was present at the conference “Building value together”, organized by our associate FNOP - National Association of Fruit and Vegetable Producers' Organizations.

InPP's executive director, António Saraiva, moderated the panel “Sustainability that generates value: The role of ESG in the future of the sector”, which included interventions from Catarina Pinto Correia (VdA), Cristina Câmara (APED), Filipa Saldanha (Crédito Agrícola), Joana Oom de Sousa (Sovena) and Rui Veríssimo Baptista (Companhia das Lezírias).

The opening session was given by Domingos dos Santos, president of FNOP and a member of CoLAB's Board of Directors of our CoLAB.

The meeting brought together producer organizations, farmers, companies, experts and political decision-makers to discuss the current challenges and look to the future of the national fruit and vegetable sector.

With the participation of national and international experts, the conference was a privileged space for sharing experiences and strategic reflection, focusing on the organization of production and the role of public policies in promoting sustainable growth.

Congratulations to FNOP for the initiative and the ability to bring together a panel of excellent speakers, making this conference a relevant and topical milestone for the sector.

Image credits: Voz do Campo magazine

FNOP Event

In viticulture, every little decision has an impact: on the soil, on the health of the plants and on the quality of the grapes that form the basis of the wine that reaches our table. The future of viticulture may depend on a single biosolution. Or a hundred. In VINNY, an ambitious European project of which InPP is a part, researchers from ten countries are looking for bioactives capable of curbing vine diseases - and, at the same time, reducing dependence on synthetic agrochemicals. What's at stake is not just science: it's the sustainability of this industry.

The aim of the VINNY project is simple but transformative: develop and implement effective, sustainable solutions and adaptable to the needs of winegrowers in various European countries, creating environmentally friendly biopesticides and biofertilizers, and advanced nano-encapsulation technologies, to reduce dependence on conventional chemicals and promote a healthier ecosystem and a better environment and a circular viticulture.

And at the heart of this mission is an essential cog in the wheel: the daily work of the researchers who search for answers invisible to the human eye - as is the case with Tiago Amaro, a researcher at InPP.

Image credits: VINNY Project

Searching for the Guardians of the Vine

The road to these new biosolutions begins in the field, with the vine. The initial work of Tiago Amaro, started in September 2024 and focuses on identifying and isolating microorganisms naturally present in the vines themselves, in samples received from partners in Portugal, Spain, Austria and Denmark.

From grapes, sticks or woody fragments, small microscopic worlds arrive in the laboratory that may contain the natural weapons needed to fighting three major threats to the vineyard, with a direct impact on farm profitability:
- A gray mold (Botrytis cinerea) and blue mold (Penicillium expansum): Fungi that cause post-harvest diseases, In the case of wine grapes, this affects the quality of the wine and makes it completely impossible to sell table grapes.
- The vine tumors: Caused by bacteria Allorhizobium vitis, This disease affects the plant in the field, causing leaf fall and reduced grape production.

Tiago Amaro, InnovPlantProtect researcher, identifying and isolating bacteria as part of the VINNY project. Image credits: InnovPlantProtect - Inês Ferreira

After isolating the microorganisms, Tiago dedicated himself to creating libraries of bacteria. What is a ‘Bacteria Library’? In the context of the investigation, a bacteria library is an organized and catalogued collection of bacteria isolated from different sources. It allows scientists to test each strain of bacteria against specific pathogens, constituting a vast catalog of potential biological ‘superheroes’ for plant protection.

This rigorous screening, which has already led to the analysis of more than 190 bacteria of this library is the first line of defense. The team selects the best candidates with the potential to be used as biological control agents against the diseases under study.

The Power of European Collaboration

What if the solution to protecting Portuguese vineyards is hidden in a Danish grape? Or in a bacterium isolated in Spain? One of the most exciting aspects of the project is its truly collaborative dimension, where researchers from ten countries are working in parallel, sharing answers, challenges and microorganisms in search of effective biosolutions for the whole of Europe.

All the solutions found will be shared, all the solutions will be tested by all the partners and it will be possible to build a ‘library of solutions’ against the various vine diseases“ emphasizes researcher Tiago Amaro.

The sharing of bacteria and extracts from different ecosystems (Portugal, Spain, Denmark and Austria) is crucial. An effective bacterium in Denmark could be the key to protecting Portuguese vineyards, and vice versa. This exchange of biological solutions, one of the innovative pillars of the project, makes it possible to exploit the microbial biodiversity beyond national borders. InPP has the fundamental role of testing, in grapes, the solutions discovered by our team as well as by other national and European partners.

This diversity of tests is a bet on the future: microorganisms that don't prove effective against vine diseases could be the solution for pathologies in other crops.

Left photo: Tiago Amaro, InPP researcher, observing a grapevine leaf, the target crop of the VINNY project, Right photo: Potted grapevine plants in the InPP greenhouse, ready to test the solutions found by the various VINNY partners. Image credits: InnovPlantProtect - Inês Ferreira

The Real Test: From the Lab to the Field

After selection in the laboratory, the next step - the formulation of the most promising bacteria - will be carried out in Portugal and Spain, at the University of Minho and the Polytechnic University of Catalonia. But it is in the field-testing phase that the greatest challenge of plant protection science lies, because even brilliant results in the laboratory can fail in the field. Formulation is the process that turns a bacterium into a product - stable, applicable and compatible with the farmer's needs.

Tiago Amaro emphasizes necessary resilience:

  • Field Uncertainty: Often, promising solutions in the laboratory or greenhouse are not as effective when applied in the field, due to environmental variables (climate, soil, etc.).
  • The Time Factor: Diseases such as Allorhizobium vitis may take a long time to develop, or the infection may not be relevant in certain years, which makes it difficult to obtain robust conclusions.
  • The Agricultural Cycle: It is necessary to test the formulation in the field during three to five consecutive years, and recording all the variations observed. With only one harvest a year, this process requires patience and persistence.

In total, from the discovery of a promising bacterium to the creation of a formulated product, proven to be effective and ready for the market, it can take around 10 years - a real test of any scientist's resilience.

Customized solutions: the new requirement of modern agriculture

The final challenge is to ensure that the tests are relevant to the producer's reality. The current trend in the agricultural sector is the search for customized solutions, adapted to the specific conditions of the farms: “There has to be a solution for every field and every farmer”, says the researcher.

This personalized approach requires more science, more rigor and more local knowledge - exactly what VINNY seeks to build.

A Europe united by science and the vine

InPP is part of this consortium, made up of 19 partners from ten countries, The project is led by the University of Minho and funded by the Horizon Europe program.

Together, they seek to answer a question that could shape the future of European viticulture: Will it be possible to find effective biosolutions for all partner countries?

The answer is still being written - in laboratories, in experimental vineyards, in fields in different climates and geographies.
And it's made up of small discoveries, many frustrations and a huge commitment to science.

Because protecting the vineyard of the future is not just a technical ambition.
It is a cultural, economic and environmental commitment.
And VINNY is helping to design that future - one microorganism at a time.

The final workshop highlighted three years of research dedicated to the early detection of pathogens in crops such as wheat and olive groves.

The project AlViGen has reached its final stretch, concluding three years of research focused on the genomic surveillance of agricultural diseases. The results now presented promise to strengthen the Alentejo agricultural sector's ability to respond to emerging phytosanitary threats.

On the day October 23rd, The final project workshop, The event brought together researchers, producers and technicians to share results and reflect on the future of genomic surveillance in Portuguese agriculture.

A pioneering genomic surveillance center

During AlViGen, the Alentejo's first genomic surveillance center, an infrastructure with capacity for early detection of diseases in strategic crops such as wheat and olive grove. This breakthrough marks a decisive step towards a more precise, sustainable and science-based agriculture.

Results and scientific contributions

Using innovative molecular tools, the project team succeeded:

  • Identify pathogenic fungi before visible symptoms appear on the plants;
  • Characterizing yellow rust strains, genetically linking them to others known at a global level;
  • Detecting resistance genes in wheat to the strains currently present in Portugal;
  • Developing diagnostic methods able to distinguish the different species of the fungus that causes gafa in olive groves.

During the workshop, the potential of the analysis of the airborne fungi community as a tool for early warning for multiple pathogens, allowing for more effective and preventive management of crop diseases.

From research to practical application

The event ended with a debate on how transform AlViGen results in a detection and warning service accessible to the agricultural sector. The initiative reflects the joint commitment between science, innovation and production, with a view to protecting national agriculture from the challenges of the future.

Partnerships and thanks

InnovPlantProtect would like to thank all the partners and funders of the project:
University of Évora, John Innes Centre, INIAV, De Prado, CERSUL, Eugénio de Almeida Foundation, Torre das Figueiras Estate, Almojanda, Malheiro Estate, Directorate-General for Food and Veterinary (DGAV), la Caixa“ Foundation”, BPI Bank e Foundation for Science and Technology (FCT).

Image credits: InnovPlantProtect - Inês Ferreira

EVENTS

Elvas collaborative laboratory distinguished by Rural Life as “the most significant investment in the last year in the agricultural and agro-industrial sector”.

“Investment that makes a mark” 2021 is the name of the prize awarded today to InnovPlantProtect by Rural Life. The award, conferred by the editorial board of the professional agribusiness magazine, recognizes the most significant investment in the last year in the agricultural and agro-industrial sector at a national level.

The choice of the collaborative laboratory (CoLab), based in Elvas, “aims to reinforce the importance of investing in research and innovation in a key area for the future of agriculture: the search for biologically-based solutions for crop protection, in a challenging context, in which it is necessary to ensure the production of food in an increasingly sustainable way,” explains Isabel Martins, director of the company. Rural Life and publications coordinator at IFE.

This award “honors the initiative of the 12 founding partners to create an innovative institution to develop biological and digital solutions for crop protection,” says Pedro Fevereiro, the CoLab's executive director, adding: “It's an incentive for InnovPlantProtect, which set up in a low-density region and managed to attract 38 highly qualified human resources to set up a modern innovation unit. It also rewards the vision of the municipality of Elvas, which embraced this challenge from the outset.”

The “Investment that Makes a Mark” 2021 award was presented during the 8th edition of the AgroIn - Annual Agribusiness Congress, organized by Rural Life/ IFE, held in the auditorium of the Faculty of Dental Medicine of the University of Lisbon.

The InPP team, like scientists around the world, concludes that new techniques can mitigate the challenges posed by climate change and the continuing increase in the world's population.

We know that in order to feed the world's population in 2050, we will have to increase food production by 70% [1]. And we also know that agriculture is under intense pressure to meet the sustainability goals of the European Green Deal and Farm to Fork Strategy, This is under pressure from climate change and the increase in pests and diseases attacking crops.

A team from InnovPlantProtect (InPP) reviewed hundreds of scientific articles published in recent years and concluded that “genome editing is an important tool for improving food security in a sustainable way and mitigating the challenges posed by global climate change and the expansion of the world's population”. The review article [2], entitled Genome editing for resistance against plant pests and pathogens, has just been published in Transgenic Research.

“The development of new tools to improve plant protection is critical in the context of current agricultural, environmental and ecological challenges,” say the researchers, adding that “various efforts in the area of genome editing have resulted in plants with potentially beneficial characteristics that can be quickly and easily applied in the field.”.

Crops are invariably exposed to pests and diseases (left). In order to speed up the plant breeding process, genome editing technologies such as CRISPR-Cas make it possible to introduce resistance mechanisms or remove susceptibility quickly and precisely (right).

The team analyzed the latest advances in genome editing with a view to improving plant protection, focusing on editing the genomes of crops, pests and pathogens based on the CRISPR-Cas technique (Nobel Prize in Chemistry in 2020). The researchers did not forget other technologies, such as host-induced gene silencing (HIGS) and the use of biocontrol agents, discussing how CRISPR-Cas can be used to accelerate the development of ecological strategies that promote sustainable agriculture in the future.

[HIGS consists of the plant producing small molecules of RNA (ribonucleic acid), which lead to the silencing of the pathogen's genes; silencing a gene means “canceling” its expression].

As genome editing does not require crossbreeding, the authors of the article point out, it avoids the introduction of unwanted characteristics through genetic linkage (a situation in which a gene with a positive characteristic is physically linked to a gene that confers negative characteristics) in improved varieties, speeding up the entire breeding process.

In addition, genome editing technologies can directly target the susceptibility genes or virulence factors of pests and pathogens, either by directly editing the genome of the pest in question, or by adding genome editing mechanisms to the plant genome, or to microorganisms that act as biocontrol agents.

Several studies have shown that the targeted inactivation of susceptibility genes through genome editing is a solid strategy for crop protection, capable of producing non-transgenic plants. The technique has been shown to be particularly effective in editing susceptibility genes to pathogenic bacteria and fungi.

“The potential impact of genome editing, and CRISPR technology in particular, on plant synthetic biology to improve resistance to pests and diseases is enormous and will have a direct effect on agricultural sustainability on a scale never seen before,” the team concludes.

Original article:

Rato, C., Carvalho, M.F., Azevedo, C. & Oblessuc, Paula. Genome editing for resistance against plant pests and pathogens. Transgenic Research (2021). https://doi.org/10.1007/s11248-021-00262-x

[1] How to Feed the World in 2050, FAO expert paper

[2] In a review article, researchers critically summarize what has already been studied and published by other scientists.

Elvas City Council promotes a visit to the works in progress at InnovPlantProtect and at the Elvense pole of the National Institute for Agrarian and Veterinary Research.

The work in progress at INIAV Elvas, including those that will accommodate the permanent facilities of the InnovPlantProtect (InPP), were visited yesterday, June 16, by a delegation that included the president of the Elvas City Council, Nuno Mocinha, the vice-president of the municipality, Cláudio Carapuça, as well as a number of council technicians, the presidents of parish councils in the municipality and representatives of the media.

InPP is an institution that wants to last for many, many years, not a “one-off project”, said the executive director of the collaborative laboratory (CoLab). Pedro February He also highlighted the fact that the research and innovation carried out here “leaves” the laboratory for the field, in the form of specific, biologically-based products and services supplied to companies, farmers and producers, among others.

Nuno Mocinha stressed the CoLab's great importance for agriculture and the region, particularly thanks to the establishment of highly qualified workers dedicated to science and technology applied in the field.

The visit took place as part of a tour promoted by the CME of some of the works underway in the municipality.