InnovPlantProtect (InPP) was present at the conference “Building value together”, organized by our associate FNOP - National Association of Fruit and Vegetable Producers' Organizations.
InPP's executive director, António Saraiva, moderated the panel “Sustainability that generates value: The role of ESG in the future of the sector”, which included interventions from Catarina Pinto Correia (VdA), Cristina Câmara (APED), Filipa Saldanha (Crédito Agrícola), Joana Oom de Sousa (Sovena) and Rui Veríssimo Baptista (Companhia das Lezírias).
The opening session was given by Domingos dos Santos, president of FNOP and a member of CoLAB's Board of Directors of our CoLAB.
The meeting brought together producer organizations, farmers, companies, experts and political decision-makers to discuss the current challenges and look to the future of the national fruit and vegetable sector.
With the participation of national and international experts, the conference was a privileged space for sharing experiences and strategic reflection, focusing on the organization of production and the role of public policies in promoting sustainable growth.
Congratulations to FNOP for the initiative and the ability to bring together a panel of excellent speakers, making this conference a relevant and topical milestone for the sector.
In viticulture, every little decision has an impact: on the soil, on the health of the plants and on the quality of the grapes that form the basis of the wine that reaches our table. The future of viticulture may depend on a single biosolution. Or a hundred. In VINNY, an ambitious European project of which InPP is a part, researchers from ten countries are looking for bioactives capable of curbing vine diseases - and, at the same time, reducing dependence on synthetic agrochemicals. What's at stake is not just science: it's the sustainability of this industry.
The aim of the VINNY project is simple but transformative: develop and implement effective, sustainable solutions and adaptable to the needs of winegrowers in various European countries, creating environmentally friendly biopesticides and biofertilizers, and advanced nano-encapsulation technologies, to reduce dependence on conventional chemicals and promote a healthier ecosystem and a better environment and a circular viticulture.
And at the heart of this mission is an essential cog in the wheel: the daily work of the researchers who search for answers invisible to the human eye - as is the case with Tiago Amaro, a researcher at InPP.
Image credits: VINNY Project
Searching for the Guardians of the Vine
The road to these new biosolutions begins in the field, with the vine. The initial work of Tiago Amaro, started in September 2024 and focuses on identifying and isolating microorganisms naturally present in the vines themselves, in samples received from partners in Portugal, Spain, Austria and Denmark.
From grapes, sticks or woody fragments, small microscopic worlds arrive in the laboratory that may contain the natural weapons needed to fighting three major threats to the vineyard, with a direct impact on farm profitability: - A gray mold (Botrytis cinerea) and blue mold (Penicillium expansum): Fungi that cause post-harvest diseases, In the case of wine grapes, this affects the quality of the wine and makes it completely impossible to sell table grapes. - The vine tumors: Caused by bacteria Allorhizobium vitis, This disease affects the plant in the field, causing leaf fall and reduced grape production.
Tiago Amaro, InnovPlantProtect researcher, identifying and isolating bacteria as part of the VINNY project. Image credits: InnovPlantProtect - Inês Ferreira
After isolating the microorganisms, Tiago dedicated himself to creating libraries of bacteria. What is a ‘Bacteria Library’? In the context of the investigation, a bacteria library is an organized and catalogued collection of bacteria isolated from different sources. It allows scientists to test each strain of bacteria against specific pathogens, constituting a vast catalog of potential biological ‘superheroes’ for plant protection.
This rigorous screening, which has already led to the analysis of more than 190 bacteria of this library is the first line of defense. The team selects the best candidates with the potential to be used as biological control agents against the diseases under study.
The Power of European Collaboration
What if the solution to protecting Portuguese vineyards is hidden in a Danish grape? Or in a bacterium isolated in Spain? One of the most exciting aspects of the project is its truly collaborative dimension, where researchers from ten countries are working in parallel, sharing answers, challenges and microorganisms in search of effective biosolutions for the whole of Europe.
“All the solutions found will be shared, all the solutions will be tested by all the partners and it will be possible to build a ‘library of solutions’ against the various vine diseases“ emphasizes researcher Tiago Amaro.
The sharing of bacteria and extracts from different ecosystems (Portugal, Spain, Denmark and Austria) is crucial. An effective bacterium in Denmark could be the key to protecting Portuguese vineyards, and vice versa. This exchange of biological solutions, one of the innovative pillars of the project, makes it possible to exploit the microbial biodiversity beyond national borders. InPP has the fundamental role of testing, in grapes, the solutions discovered by our team as well as by other national and European partners.
This diversity of tests is a bet on the future: microorganisms that don't prove effective against vine diseases could be the solution for pathologies in other crops.
Left photo: Tiago Amaro, InPP researcher, observing a grapevine leaf, the target crop of the VINNY project, Right photo: Potted grapevine plants in the InPP greenhouse, ready to test the solutions found by the various VINNY partners. Image credits: InnovPlantProtect - Inês Ferreira
The Real Test: From the Lab to the Field
After selection in the laboratory, the next step - the formulation of the most promising bacteria - will be carried out in Portugal and Spain, at the University of Minho and the Polytechnic University of Catalonia. But it is in the field-testing phase that the greatest challenge of plant protection science lies, because even brilliant results in the laboratory can fail in the field. Formulation is the process that turns a bacterium into a product - stable, applicable and compatible with the farmer's needs.
Tiago Amaro emphasizes necessary resilience:
Field Uncertainty: Often, promising solutions in the laboratory or greenhouse are not as effective when applied in the field, due to environmental variables (climate, soil, etc.).
The Time Factor: Diseases such as Allorhizobium vitis may take a long time to develop, or the infection may not be relevant in certain years, which makes it difficult to obtain robust conclusions.
The Agricultural Cycle: It is necessary to test the formulation in the field during three to five consecutive years, and recording all the variations observed. With only one harvest a year, this process requires patience and persistence.
In total, from the discovery of a promising bacterium to the creation of a formulated product, proven to be effective and ready for the market, it can take around 10 years - a real test of any scientist's resilience.
Customized solutions: the new requirement of modern agriculture
The final challenge is to ensure that the tests are relevant to the producer's reality. The current trend in the agricultural sector is the search for customized solutions, adapted to the specific conditions of the farms: “There has to be a solution for every field and every farmer”, says the researcher.
This personalized approach requires more science, more rigor and more local knowledge - exactly what VINNY seeks to build.
A Europe united by science and the vine
InPP is part of this consortium, made up of 19 partners from ten countries, The project is led by the University of Minho and funded by the Horizon Europe program.
Together, they seek to answer a question that could shape the future of European viticulture: Will it be possible to find effective biosolutions for all partner countries?
The answer is still being written - in laboratories, in experimental vineyards, in fields in different climates and geographies. And it's made up of small discoveries, many frustrations and a huge commitment to science.
Because protecting the vineyard of the future is not just a technical ambition. It is a cultural, economic and environmental commitment. And VINNY is helping to design that future - one microorganism at a time.
The final workshop highlighted three years of research dedicated to the early detection of pathogens in crops such as wheat and olive groves.
The project AlViGen has reached its final stretch, concluding three years of research focused on the genomic surveillance of agricultural diseases. The results now presented promise to strengthen the Alentejo agricultural sector's ability to respond to emerging phytosanitary threats.
On the day October 23rd, The final project workshop, The event brought together researchers, producers and technicians to share results and reflect on the future of genomic surveillance in Portuguese agriculture.
A pioneering genomic surveillance center
During AlViGen, the Alentejo's first genomic surveillance center, an infrastructure with capacity for early detection of diseases in strategic crops such as wheat and olive grove. This breakthrough marks a decisive step towards a more precise, sustainable and science-based agriculture.
Results and scientific contributions
Using innovative molecular tools, the project team succeeded:
Identify pathogenic fungi before visible symptoms appear on the plants;
Characterizing yellow rust strains, genetically linking them to others known at a global level;
Detecting resistance genes in wheat to the strains currently present in Portugal;
Developing diagnostic methods able to distinguish the different species of the fungus that causes gafa in olive groves.
During the workshop, the potential of the analysis of the airborne fungi community as a tool for early warning for multiple pathogens, allowing for more effective and preventive management of crop diseases.
From research to practical application
The event ended with a debate on how transform AlViGen results in a detection and warning service accessible to the agricultural sector. The initiative reflects the joint commitment between science, innovation and production, with a view to protecting national agriculture from the challenges of the future.
Partnerships and thanks
InnovPlantProtect would like to thank all the partners and funders of the project: University of Évora, John Innes Centre, INIAV, De Prado, CERSUL, Eugénio de Almeida Foundation, Torre das Figueiras Estate, Almojanda, Malheiro Estate, Directorate-General for Food and Veterinary (DGAV), la Caixa“ Foundation”, BPI Bank e Foundation for Science and Technology (FCT).
Insect vector of Xylella is attracted or repelled by different aromatic plants depending on the sex of the adult and its distance from the source of essential oils.
Lavandula angustifolia Photo: JLPC/ Wiki
It was already known that the nefarious Xylella fastidiosa “liked” Lavandula spp.; after all, it was in a lavender plant that the presence of the bacterium in Portugal was confirmed for the first time in January 2019. A team of scientists has since concluded that the males of the insect vector of the Xylella in Europe are attracted to the essential oils of lavender over long distances.
“Sustainable vector control is an essential part of pest management strategies.”, remember those responsible for the European XF-ACTORS project. The results of this study could contribute to the development of innovative approaches and tools to control the insect, as alternatives to the use of synthetic pesticides Philaenus spumarius, vector of Xylella fastidiosa in Europe - essential to control the spread of the bacteria.
Lavandula angustifolia Photo: Norbert Nagel/ Wiki
The team found that the peripheral olfactory system of the P. spumarius captures the volatile organic compounds present in the essential oils of pelargonium (also known as geranium or sardinia). Pelargonium graveolens, from Cymbopogon nardus (a relative of lemongrass), which produces citronella, and the Lavandula angustifolia (before officinalis), lavender. But the effect of this detection depends on the “dose” and the distance.
At any distance, both lavender and pelargonium repelled the females. Males, on the other hand, were attracted to geranium and repelled by citronella. At close range, both lavender and citronella were repellent to both females and males.
You can consult the original scientific article here.
In celebration of the International Day of Women and Girls in Science, some of InnovPlantProtect's women researchers share the reasons they became scientists.
“I became a scientist for the thrill of being able to discover something new every day and a plant scientist in particular with the dream of producing plants resistant to pathogens, avoiding the need for chemical pesticides. A win-win solution to reduce the environmental impacts of agriculture and the production costs to small farmers.”
Cristina Azevedo, PhD researcher, Head of the New Biopesticides Department
Cristina Azevedo
Cláudia Rato da Silva
Manisha Sirsat
“Ever since I was a child, I always felt drawn to nature, particularly at the beach, where I would explore sea life, but also at my grandparents’ vegetable garden, where I loved to play. The fact that my mother is really passionate about nature, particularly about plants and birds, also played a role when I decided to apply for Biology. On top of that, there's my desire to understand the world around me; being a scientist allows me to ask questions and work towards an answer, and that moment when you discover something new in the lab is still beyond thrilling.”
Cláudia Rato da Silva, PhD researcher, Protection of Specific Crops Department
“I have had a passion for technology and science since childhood, so I decided to pursue my career in a computer science discipline in which Artificial Intelligence is one of the great research fields, which could help to solve complex problems of nearly every field. ‘The human brain cannot deal with terabytes but Machine Learning can!’ Hence I was inspired to do research in this field.”
Manisha Sirsat, PhD researcher, Department of Data Management and Risk Analysis
Cláudia Almeida Silva
Cátia Patrício
Ana Cláudia Silva
“When I was younger, I used to ask my parents ‘Why this happens?’ or ‘Why this works the way it does?’ Most of the questions were ‘Why?’ and my parents didn't know how to answer, so they encouraged me to go look for it. From then on, I didn't ask my parents anymore. I would search for the answer. That feeling did not disappear as I grew up. So, during my school years, I always searched for the answer to the question ‘Why?’. When I was in 12th grade, I had to choose my college degree and it was very difficult to choose between all the available courses, but I knew that it would be related to science, because 99% of the questions that you ask, Science can answer it. And that was the moment that I decided to become a scientist - to search for the answers to my questions. Science is a part of our life, Science is in everything, with Science you can change the world.”
Cláudia Almeida Silva, MSc researcher, Department of New Formulations and Matrices for the Application of Biopesticides
“I decided to become a scientist when my father got sick, and I knew that I should contribute to science to help answer the questions that still had no answers. This profession showed me that there are many things that we already know but, on the other hand, there is much more that we need to discover and learn. The idea that small discoveries that you can make can lead to the solution of big problems is gratifying to me.”
Cátia Patrício, MSc Researcher, Department of New Biopesticides
“As an undergraduate, I started collaborating in a Nematology laboratory at the University where I was studying. There began my passion for science, and particularly for phytopathology. Since then, I have been gaining more knowledge and working in this topic, and it is a great pleasure for me to be able to keep on following this career at the InnovPlantProtect CoLab, with such a prodigious team of women and men scientists.”
Ana Cláudia Silva, MSc Researcher, Department of New Biopesticides
Maria Miguel Pires
Diana Pimentel
Jordana Branco
“I didn't always want to become a scientist. One thing I did know was that I had been forever fascinated with my surroundings, nature at its finest. And there was this random driving force that made me question things, as although I felt an overwhelming need to pursue all the answers. Why is everything the way it is? Why is that leaf falling from the tree? Questioning, and an overall endless curiosity, that's what made me become ‘sort-of-a-scientist’”.
Maria Miguel Pires, MSc Researcher, Department of New Biopesticides
“Since childhood, I have always been fascinated with nature, how plants survive on rainy days, how the dough rises, how the grape juice that I could drink turns into something that I was not allowed to... at the end of the day, how life makes sense! So, I wanted to know more and more, and that curiosity made me pursue life sciences.”
Diana Pimentel, PhD researcher/ Biostatistics Technician, Department of Data Management and Risk Analysis
“Isn't it fascinating how the tiniest organisms can affect a whole ecosystem? And, even more so, to understand how they work and how we can fight or improve them? This feeling was what made me pursue a scientific career. Science is non-stop challenges but is also non-stop knowledge and progress.”
Jordana Branco, MSc researcher, Department of New Biopesticides
Diana Sousa
“I have always loved nature and helping people, in order to always contribute to a more sustainable world and better health for all people. I confess that I was also fascinated by the investigation, due to CSI. Ahahah And that's why I became a scientific researcher, always ready to promote a better world.”
Diana Sousa, MSc researcher, Department Protection of Specific Crops
XfSTOP aims to develop a solution to protect olive groves against the bacterium Xylella fastidiosa. The project is led by InnovPlantProtect's New Biopesticides Department - Department 1, headed by researcher Cristina Azevedo.
The team is now starting laboratory work, with the aim of trying to isolate peptides (small amino acids) that inhibit the growth of the bacterial pathogen Xylella fastidiosa. “We have established collaboration with researchers in different countries, through which we have obtained genomic DNA from different isolates [strains] of Xylella, and we are in the process of isolating the genes that code for the virulence proteins against which we want to select the peptides,” explains Cristina Azevedo.
Isolation of Xylella fastidiosa (strain LM10)
The director of Dep. 1 said that the team is also “starting to isolate bacterial endophytes [organisms that live inside a host] present in the xylem of olive varieties grown in the Alentejo region (Cobrançosa and Arbequina) and of an Italian variety that is resistant to Xylella (Leccino), from the Herdade de Reguengo, belonging to INIAV, Elvas center”.
The project's acronym, XfSTOP, stands for “Integrated approach to disease management for the biocontrol of Xylella fastidiosa in olive trees”. The olive tree is of enormous economic importance in Portugal, particularly in the Alentejo. The biopesticide to be developed will use a microorganism capable of coexisting in the same environment as the olive tree. Xylella, The plant will then produce a biological compound capable of eliminating the bacteria. This mechanism will be sustainable, environmentally friendly and non-toxic.
Português 
English